L1287

Sigma

 

Lectin from Dolichos biflorus (horse gram)

peroxidase conjugate, lyophilized powder

Synonym:Dolichos biflorus agglutinin, DBA

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Description

Biochem/physiol ActionsDBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity,1 and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues.2 Conjugates are prepared from affinity purified lectin.
Preparation NotePrepared from peroxidase type VI (P8375) using a modification of the method of O'Sullivan3, which favors low molecular weight conjugates.
Unit DefinitionOne unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical formContains sodium citrate

Properties

formlyophilized powder
peroxidase activity10-30 units/mg protein
compositionProtein, ~90% biuret
conjugateperoxidase conjugate
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

References

Cited Reference1. Bird, G. W. G. Blood 21, 366, (1970)
 2. Etzler, M. E., Kabat, E. A. Biochemistry 9, 869, (1970)
 3. O'Sullivan, M. J., et al. FEBS Lett. 95, 311, (1978) Abstract
referenceEtzler, M. E. Meth. Enzymol. 28, 340, (1972)